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Differing reflections of paediatric classical Hodgkin’s lymphoma on local and distant immunological microenvironments: a flow cytometric study
  1. Süreyya Savaşan,
  2. Batool AlQanber,
  3. Meret Henry,
  4. Steven Buck,
  5. Manisha Gadgeel
  1. Hematology/Oncology, Children’s Hospital of Michigan, Wayne State University, Detroit, Michigan, USA
  1. Correspondence to Dr Süreyya Savaşan, Hematology/Oncology, Children's Hospital of Michigan, Detroit, MI 48201, USA; ssavasan{at}


Aims To compare immunological microenvironments in local and distant lymphoid tissues in Hodgkin’s lymphoma (HL) in children.

Methods We have analysed diagnostic bone marrow (BM) samples in 22 and corresponding involved lymph node (LN) in eight and peripheral blood (PB) in eight cases of HL by flow cytometry and sought correlations with clinical features retrospectively.

Results While there were significant differences in lymphocyte compositions of BM and LN tissues, the distribution of lymphocyte subsets mimicked each other in BM and PB. CD8-positive cytotoxic T cells predominate the bone marrow in contrast to CD4-positive helper T cells in LN tissue with corresponding CD4/CD8 ratios (0.85 and 5.3, respectively; p=0.002). Additionally, T-large granular lymphocytes population was much higher in BM in comparison to LN tissue (10.5% vs 4.5%; p=0.036).

Conclusions Local immunological microenvironment appears to be highly influenced by HL tumour cells and distant site lymphocyte composition reflects immune response to control the neoplastic process.

  • Hodgkin lymphoma
  • immunological microenvironment
  • lymph node
  • bone marrow
  • flow cytometry

Statistics from


  • Handling editor Mary Frances McMullin.

  • Contributors SS planned the study, analysed the results, drafted the manuscript and prepared the table. BAQ reanalysed the flow cytometric tests and entered patient data. MG prepared the figure. BAQ, MH, SB and MG edited the manuscript.

  • Funding This study is partially supported by funds from Children’s Hospital of Michigan Foundation.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval Human Investigation Committee at Wayne State University approval was obtained to review cases, which had flow cytometric analysis carried out at Hematology/Oncology Flow Cytometry Laboratory.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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