Development of a qualitative real-time RT-PCR assay for the detection of SARS-CoV-2: a guide and case study in setting up an emergency-use, laboratory-developed molecular microbiological assay

Melis N Anahtar; Bennett M Shaw; Damien Slater; Elizabeth H Byrne; Yolanda Botti-Lodovico; Gordon Adams; Stephen F Schaffner; Jacqueline Eversley; Graham E G McGrath; Tasos Gogakos; Jochen Lennerz; Hetal Desai Marble; Lauren L Ritterhouse; Julie M Batten; N Zeke Georgantas; Rebecca Pellerin; Sylvia Signorelli; Julia Thierauf; Molly Kemball; Christian Happi; Donald S Grant; Daouda Ndiaye; Katherine J Siddle; Samar B Mehta; Jason B Harris; Edward T Ryan; Virginia M Pierce; Regina C LaRocque; Jacob E Lemieux; Pardis C Sabeti; Eric S Rosenberg; John A Branda; Sarah E Turbett

doi:10.1136/jclinpath-2020-207128

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Development of a qualitative real-time RT-PCR assay for the detection of SARS-CoV-2: a guide and case study in setting up an emergency-use, laboratory-developed molecular microbiological assay

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