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Development of a qualitative real-time RT-PCR assay for the detection of SARS-CoV-2: a guide and case study in setting up an emergency-use, laboratory-developed molecular microbiological assay

Authors

  • Melis N Anahtar Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Bennett M Shaw Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Damien Slater Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Elizabeth H Byrne Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Yolanda Botti-Lodovico Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Gordon Adams Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Stephen F Schaffner Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Jacqueline Eversley Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Graham E G McGrath Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Tasos Gogakos Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Jochen Lennerz Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Hetal Desai Marble Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Lauren L Ritterhouse Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Julie M Batten Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • N Zeke Georgantas Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Rebecca Pellerin Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Sylvia Signorelli Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Julia Thierauf Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA Department of Otorhinolaryngology, University Hospital Heidelberg, Heidelberg, Baden-Württemberg, Germany PubMed articlesGoogle scholar articles
  • Molly Kemball Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Christian Happi Department of Biological Sciences, Redeemer's University, Ede, Osun, Nigeria African Center of Excellence for Genomics of Infectious Diseases, Redeemer's University, Ede, Osun, Nigeria PubMed articlesGoogle scholar articles
  • Donald S Grant Viral Hemorrhagic Fever Program, Kenema Government Hospital, Kenema, Sierra Leone College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown, Sierra Leone PubMed articlesGoogle scholar articles
  • Daouda Ndiaye African Center of Excellence for Genomics of Infectious Diseases, Redeemer's University, Ede, Osun, Nigeria Department of Mycology and Pharmacology, Universite Cheikh Anta Diop, Dakar, Senegal PubMed articlesGoogle scholar articles
  • Katherine J Siddle Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Samar B Mehta Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Jason B Harris Department of Pediatrics, Massachusetts General Hospital for Children, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Edward T Ryan Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Virginia M Pierce Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA Department of Pediatrics, Massachusetts General Hospital for Children, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Regina C LaRocque Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Jacob E Lemieux Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Pardis C Sabeti Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA Immunology and Infectious Disease, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA Howard Hughes Medical Institute, Chevy Chase, Maryland, USA PubMed articlesGoogle scholar articles
  • Eric S Rosenberg Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • John A Branda Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  • Sarah E Turbett Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA PubMed articlesGoogle scholar articles
  1. Correspondence to Dr Pardis C Sabeti, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA; pardis{at}broadinstitute.org; Dr Jacob E Lemieux; lemieux{at}broadinstitute.org; Dr John A Branda; Branda.John{at}mgh.harvard.edu; Dr Sarah E Turbett; Turbett.Sarah{at}mgh.harvard.edu
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Citation

Anahtar MN, Shaw BM, Slater D, et al
Development of a qualitative real-time RT-PCR assay for the detection of SARS-CoV-2: a guide and case study in setting up an emergency-use, laboratory-developed molecular microbiological assay
Journal of Clinical Pathology 2021;74:496-503.

Publication history

  • Received September 21, 2020
  • Revised January 20, 2021
  • Accepted January 30, 2021
  • First published May 28, 2021.
Online issue publication 
December 27, 2022

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. http://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.