Aims To assess the utility of a three-antibody immunohistochemistry panel to classify muscle invasive bladder cancers (MIBCs) in correlation with morphological features and p53 status.
Methods A retrospective review of 243 chemotherapy naïve MIBC cystectomy specimens was performed to assess morphological features. A tissue microarray was sequentially stained with CK5/6, GATA-3 and p16. Subgroups were assigned as basal-like (CK5/6+, GATA3−) and luminal (CK5/6−, GATA3+), with the latter subdivided into genomically unstable (GU, p16+) and urothelial like (Uro, p16−) subgroups. p53 staining was assessed as abnormal/wild type. Cases from the The Cancer Genome Atlas (TCGA) portal were assessed as external validation.
Results We identified 78.8% luminal, 21.2% basal cases within our cohort and 63.4% luminal, 36.6% basal in the TCGA dataset. Divergent differentiation (p<0.001) was significantly associated with basal-subtype cases in both cohorts. Within the luminal subgroup (n=186), 81 cases were classified as GU and 105 as Uro. Abnormal p53 staining was noted in 48.0% of basal, 80.2% GU and 38.1% Uro cases. Further, basal-subtype tumours significantly correlated with disease-specific death compared with Uro cases in multivariate survival analysis.
Conclusions This retrospective study demonstrates the potential utility of a three-antibody immunohistochemistry panel to differentiate luminal and basal MIBC.
- urinary bladder
Data availability statement
Data are available on reasonable request.
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Handling editor Runjan Chetty.
Contributors MRD designed the study. AH, BX and MRD gathered the immunohistochemistry data. EO-M, AH, SKL, DV, BX and MRD reviewed and interpreted the data. EO-M performed the statistical analysis. EO-M, AH, SKL, DV, BX and MRD contributed to drafting and review of the manuscript, and to approval of the final version.
Funding Sunnybrook Health Sciences Centre departmental Educational Grant.
Competing interests None declared.
Provenance and peer review Not commissioned; externally peer reviewed.