DNA mismatch repair complex is involved in the maintenance of DNA stability. In the recent years, a plethora of technical approaches for microsatellite instability (MSI) analysis emerged. Here, we review the results of our MSI status evaluation by adopting a customised workflow on microfluidic system obtained in 4 years of diagnostic routine practice. Data from MSI status were retrieved from our institutional archive covering the period from January 2017 to December 2021. Microfluidic analysis was carried out on microfluidic platform. Results were inspected with a proprietary software. Overall, microsatellite stability (MSS) and MSI-high (MSI-H) profile was detected in n=423/458 (92.36%) and n=35/458 (7.64%) patients with metastatic CRC (mCRC), respectively. In addition, n=78/86 (90.70%) and n=8/86 (9.30%) patients without CRC showed an MSS and MSI-H profile. This review highlights the suitability of microfluidic approach in patients with cancer for MSI testing.
- pathology, molecular
- molecular biology
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Handling editor Runjan Chetty.
Twitter @PasqualePisapia, @UmbertoMalapel1
Contributors Conceptualisation: UM; methodology: all authors; software: all authors; validation: all authors; formal analysis: all authors; investigation: all authors; resources: all authors; data curation: all authors; writing—original draft preparation: GR, FP, PPi, UM; writing—review and editing: all authors; visualisation: all authors; supervision: GT, UM; project administration: GT, UM; funding acquisition: GT.
Funding Monitoraggio ambientale, studio ed approfondimento della salute della popolazione residente in aree a rischio—In attuazione della D.G.R. Campanian.180/2019. POR Campania FESR 2014–2020 Progetto “Sviluppo di Approcci Terapeutici Innovativi per patologie Neoplastiche resistenti ai trattamenti—SATIN”.
Competing interests PPi has received personal fees as speaker bureau from Novartis, unrelated to the current work. EV has received personal fees (as consultant and/or speaker bureau) from Diaceutics, AstraZeneca unrelated to the current work. MF has received personal fees (as consultant and/or speaker bureau) from Roche, Diaceutics, GSK and Astellas Pharma and research grants from Astellas Pharma, QED Therapeutics and Macrophage Pharma, unrelated to the current work. GT reports personal fees (as speaker bureau or advisor) from Roche, MSD, Pfizer and Bayer, unrelated to the current work. PG received personal fees (as consultant and/or speaker bureau) from Eli Lilly, AstraZeneca, Pfizer, Novartis, Boehringer Ingelheim, Roche and MSD, unrelated to the current work. GT reports personal fees (as speaker bureau or advisor) from Roche, MSD, Pfizer, Boehringer Ingelheim, Eli Lilly, BMS, GSK, Menarini, AstraZeneca, Amgen and Bayer, unrelated to the current work. UM has received personal fees (as consultant and/or speaker bureau) from Boehringer Ingelheim, Roche, MSD, Amgen, Thermo Fisher Scientific, Eli Lilly, Diaceutics, GSK, Merck and AstraZeneca, Janssen, Diatech, Novartis, Hedera unrelated to the current work. The other authors have nothing to disclose.
Provenance and peer review Not commissioned; internally peer reviewed.
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