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Evaluation of disc susceptibility tests performed directly from positive blood cultures
  1. Thean Yen Tan (thean_yen_tan{at}
  1. Changi General Hospital, Singapore
    1. Siew Yong Ng (siew_yong_ng{at}
    1. Changi General Hospital, Singapore
      1. Lee Ling Kwang (microlab{at}
      1. Changi General Hospital, Singapore


        Aims: The aim was to evaluate the accuracy of direct disc susceptibility testing performed from positive BACTEC™ blood culture vials, using a pre-determined dilution protocol. Results were compared to those obtained by standard disc susceptibility testing according to CLSI methods.

        Methods: Direct susceptibility testing was performed from 432 positive blood culture vials, generating 3829 antibiotic-organism results.

        Results: When results were compared to the reference method, no very major error was detected. One (0.03%) major error and 89 (2.3%) minor errors were found. Error rates by organism-group ranged from 1.3% for Pseudomonas aeruginosa to 8.2% for beta-hemolytic streptococci.

        Conclusions: Direct susceptibility testing provided accurate susceptibility results for most organism-antibiotic combinations, with the exception of the beta-hemolytic streptococci.

        • bacteremia
        • disc diffusion
        • susceptibility testing

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