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TP53 and DNA-repair gene polymorphisms genotyping as a low-cost lung adenocarcinoma screening tool
  1. Milena Cavic1,
  2. Jelena Spasic2,
  3. Ana Krivokuca1,
  4. Ivana Boljevic1,
  5. Mira Kuburovic1,
  6. Davorin Radosavljevic2,
  7. Radmila Jankovic1
  1. 1 Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia
  2. 2 Clinic for Medical Oncology, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia
  1. Correspondence to Dr Milena Cavic, Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade 11000, Serbia; milena.cavic{at}ncrc.ac.rs

Abstract

Aim TP53 and DNA repair polymorphisms have been proposed as cancer risk factors. This study evaluated the usability of TP53 Arg72Pro single-nucleotide polymorphism, X RCC1 Arg399Gln and RAD51 G135C as a low-cost lung adenocarcinoma screening tool.

Patients and methods This case–control study included 78 atients with lung adenocarcinoma and 79 healthy matched controls. TP53, XRCC1 and RAD51 genotyping was done by PCR followed by restriction length polymorphism. Descriptive analyses included genotype and allelic frequencies and deviations of the frequencies from those expected under Hardy-Weinberg equilibrium were assessed using the χ2 test. The OR and 95% CIs were calculated as an estimate of relative risk, with significance set at p value <0.05.

Results The TP53 codon 72 Pro allele and the XRCC1 codon 399 Arg allele in a homozygous state were associated with lung adenocarcinoma (p=0.037; OR (95%  CI) 2.42 (1.10 to 5.31)), that is, p=0.037; OR (95%  CI) 2.16 (1.08 to 4.33), respectively. Also, carriers of the TP53 codon 72 Pro allele and the XRCC1 codon 399 ArgArg genotype older than 50 showed an even higher risk of developing lung adenocarcinoma (p=0.03 in both cases).

Conclusions The TP53 codon 72 Arg allele and XRCC1 codon 399 Gln allele are likely to have a protective effect against lung adenocarcinoma, especially in individuals older than 50 years of age. XRCC1 and TP53 genotyping might be a useful low-cost tool for evaluating individual lung cancer risk, leading to earlier detection and management of this disease.

  • cancer screening
  • lung adenocarcinoma
  • single nucleotide polymorphism
  • TP53
  • XRCC1

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Footnotes

  • Handling editor Runjan Chetty.

  • Contributors MC, JS, DR and RJ conceived and designed the study. MC, AK, IB and MK performed the experiments. MC and JS analysed the data. All authors wrote the paper and approved the final version. All authors agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

  • Funding This study was supported by a grant from the Ministry of Education and Science of the Republic of Serbia (grant no. III41026).

  • Competing Interests statement: None declared.

  • Patient consent Not required.

  • Ethics approval The Ethical Committee of the Institute for Oncology and Radiology of Serbia.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement There are no additional unpublished data from the study.

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