Article Text

other Versions

Download PDFPDF
Core needle biopsy wash as a tool for acquiring additional diagnostic material for laboratory testing
  1. Wilfrido Mojica1,
  2. Katherine Cwiklinski2,
  3. Xiaobing Jin1,
  4. Weiguo Liu3,
  5. Donald Yergeau4
  1. 1Pathology and Anatomical Sciences, University at Buffalo, Buffalo, New York, USA
  2. 2Medicine, University at Buffalo, Buffalo, New York, USA
  3. 3Pathology, New York University Medical Center, New York City, New York, USA
  4. 4NYS Center of Excellence in Bioinformatics and Life Sciences, UB Genomics and Bioinformatics Core, Buffalo, New York, USA
  1. Correspondence to Dr Wilfrido Mojica, Pathology and Anatomical Sciences, University at Buffalo, Buffalo, NY 14203, USA; mojica{at}


Aims To determine if a simple prewash step added to the processing workflow of tissue procurement by a core needle biopsy device will recover enough cells to expand the laboratory testing armamentarium.

Methods Tissue was obtained from unfixed resection specimens using a core needle device and washed in a buffered solution before fixation. This creates a liquid aliquot from which dislodged cells can be kept and separated from the tissue specimen, the latter of which can then undergo traditional formalin-fixed, paraffin-embedded processing.

Results Cells dislodged from the tissue during the biopsy procedure are recoverable, are representative of the tissue section and of sufficient quantities for additional laboratory testing.

Conclusions The core needle biopsy wash is an under-recognised and underutilised approach to extending the diagnostic capabilities of the limited amount of targeted material obtained during this common procedure. The ability to recover supplemental amounts of diagnostic material yields great potential as a substrate for a multitude of current and developing laboratory assays.

  • diagnostic techniques and procedures
  • DNA
  • image-guided biopsy
  • methods
  • pathology
  • surgical

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.


  • Handling editor Runjan Chetty.

  • Contributors WM: conceptualisation, methodology, data collection, data analysis, writing (draft, editing, review). KC: data collection, data analysis, writing (editing, review). XJ: data analysis, writing (editing, review). WL: data collection, data analysis, writing (editing, review). DY: data collection and analysis.

  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval Permission to perform this study was granted by the University at Buffalo Institutional Review Board.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement Data are available upon reasonable request.