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Altered cytokeratin 5 expression in breast lobular myoepithelial cells
  1. Anqi Li1,
  2. Miao Ruan1,
  3. Xiaochun Fei1,
  4. Haimin Xu1,
  5. Shijie Deng1,
  6. Rui Bi2,3,
  7. Wentao Yang2,3,
  8. Lei Dong1
  1. 1Department of Pathology, Shanghai Jiao Tong University Medical School Affiliated Ruijin Hospital, Shanghai, China
  2. 2Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, China
  3. 3Department of Oncology, Shanghai Medical College of Fudan University, Shanghai, China
  1. Correspondence to Dr Lei Dong, Department of Pathology, Shanghai Jiao Tong University Medical School Affiliated Ruijin Hospital, Shanghai, China; dl11968{at}rjh.com.cn; Dr Wentao Yang, Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, China; yangwt2000{at}163.com

Abstract

Aims Cytokeratin 5 (CK5) is a surrogate maker of progenitor cells and early glandular and myoepithelial cells (MECs) in the breast, and CK5 expression in breast MECs varies from ducts to lobules, and from normal to diseased tissue. However, the mechanisms underlying immunophenotypic alterations of CK5 expression in MECs remain unclear.

Methods CK5 expression in MECs of 20 normal breast samples, 58 ductal carcinoma in situ (DCIS; including 21 DCIS with extensive lobular involvement), 11 atypical ductal hyperplasia (ADH), 18 non-invasive lobular neoplasia consisting of 11 atypical lobular hyperplasia (ALH) and 7 lobular carcinoma in situ (LCIS), 20 cystic lobules and 10 usual ductal hyperplasia (UDH) involving lobules were observed to evaluate the effects of contact with benign hyperplastic or cancerous luminal cells and pressure of dilated glands on CK5 expression.

Results CK5 expression in normal ductal MECs was exclusively positive, whereas most normal lobular MECs were negative. In DCIS, cancerous ducts were primarily surrounded by CK5-positive MECs (91.0%), as were lobular acini involved by DCIS (89.2%), while the remaining normal acini maintained CK5-negative. CK5-positive MECs were found in 57.5% of acini in ALH and were more prevalent in LCIS (70.7%). CK5 expression was occasionally positive in both cystic lobules (16.7%) and lobules involved by UDH (14.3%), while an increase of CK5-positive MECs was found in ADH (38.2%).

Conclusions These results suggest that CK5 expression in lobular MECs may be altered by contact with cancerous luminal cells rather than benign hyperplastic luminal cells or pressure from dilated glands.

  • Breast Neoplasms
  • Breast Diseases
  • IMMUNOPHENOTYPING

Data availability statement

Data are available on reasonable request. The data are available on request on the following email address: laq12306@rjh.com.cn.

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Data availability statement

Data are available on reasonable request. The data are available on request on the following email address: laq12306@rjh.com.cn.

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Footnotes

  • AL and MR are joint first authors.

  • Handling editor L C Collins.

  • Collaborators None.

  • Contributors Work design (AL, MR, XF, RB, LD and WY). Data Collection and drafting the article (AL, MR and LD). Data statistics and analysis (AL, MR, XF, SD and RB). Revision of the article (LD and WY). LD accepts full responsibility for the work and/or the conduct of the study, had access to the data, and controlled the decision to publish.

  • Funding This work was supported by the Science and Technology Commission of Shanghai Municipality (No. 19441904900).

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.