PT  - JOURNAL ARTICLE
AU  - Cheng-Chih Huang
AU  - Jenn-Ren Hsiao
AU  - Ming-Wei Yang
AU  - Yuan-Hua Wu
AU  - Keng-Fu Hsu
AU  - Yao Chang
AU  - Chaio-Wei Chen
AU  - Sen-Tien Tsai
AU  - Hsuan-Pei Wei
AU  - Ying-Tai Jin
TI  - Human papilloma virus detection in neoplastic and non-neoplastic nasopharyngeal tissues in Taiwan
AID  - 10.1136/jcp.2010.087742
DP  - 2011 Jul 01
TA  - Journal of Clinical Pathology
PG  - 571--577
VI  - 64
IP  - 7
4099  - http://jcp.bmj.com/content/64/7/571.short
4100  - http://jcp.bmj.com/content/64/7/571.full
SO  - J Clin Pathol2011 Jul 01; 64
AB  - Background Human papilloma virus (HPV) has been implicated in the carcinogenesis and prognosis of certain head and neck cancers. Whether it also has a role in the pathogenesis of nasopharyngeal carcinoma (NPC) in Taiwan is unclear.Methods Detection and genotyping of HPVs were performed in 43 primary NPCs (one WHO-I and 42 WHO-II/III) and 40 nasopharyngeal controls using PCR-based HPV genotyping arrays. Localisation of high-risk HPV and Epstein–Barr virus genomes was performed in another 46 primary NPCs (five WHO-I and 41 WHO-II/III) and seven paired metastatic WHO-II/III NPCs using in situ hybridisation.Results In the HPV genotyping cohort, oncogenic HPVs were detected equally in WHO-II/III NPCs (31%, 13/42) and nasopharyngeal controls (35%, 14/40). Tumour high-risk HPV status did not correlate with the prognosis of patients with NPC. In the high-risk HPV in situ hybridisation cohort, 14 (88%) of the 16 oncogenic HPV-positive WHO-II/III NPCs showed a unique cytoplasmic/perinuclear staining pattern, which is distinct from the typical dot/punctate nuclear staining pattern indicating HPV genome integration. In addition, oncogenic HPVs were not always retained in NPC cells during the process of metastasis.Conclusions This study does not support an association between oncogenic HPV and the carcinogenesis or prognosis of WHO-II/III NPCs in Taiwan.