RT Journal Article
SR Electronic
T1 Quantitative separation of radioactive sterols and bile acids in human faeces
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 105
OP 109
DO 10.1136/jcp.18.1.105
VO 18
IS 1
A1 B. Lewis
A1 N. B. Myant
YR 1965
UL http://jcp.bmj.com/content/18/1/105.abstract
AB A method is described for the quantitative separation of radioactive bile acids and neutral sterols in human faeces on a potassium hydroxide-treated silicic acid column. Results obtained with faecal extracts containing metabolites derived from radioactive cholesterol, cholic acid, and chenodeoxycholic acid show that the efficiency of extraction is at least 96% and that mutual contamination between the bile-acid and sterol fractions is between 0·7 and 3·8%. The method gives better results than one based on separation of the faecal steroids into saponifiable and unsaponifiable material.