PT  - JOURNAL ARTICLE
AU  - Herbert, Victor
TI  - Aseptic addition method for <em>Lactobacillus casei</em> assay of folate activity in human serum
AID  - 10.1136/jcp.19.1.12
DP  - 1966 Jan 01
TA  - Journal of Clinical Pathology
PG  - 12--16
VI  - 19
IP  - 1
4099  - http://jcp.bmj.com/content/19/1/12.short
4100  - http://jcp.bmj.com/content/19/1/12.full
SO  - J Clin Pathol1966 Jan 01; 19
AB  - An `aseptic addition' method is described for microbiological assay with Lactobacillis casei of folate activity in human serum. It has the following advantages over the previously reported `standard' method. 1 The manipulations involved in the assay are halved, by deleting autoclaving of serum in buffers. 2 The use of 1 g.% ascorbate better preserves serum folates than the lower amounts of ascorbate which are the maximum quantities usable in the standard methods. 3 Only 0·3 ml. of serum is required (0·1 ml. for one sample; 0·2 ml. for its duplicate).