TY - JOUR T1 - A rapid micro method for recording red cell osmotic fragility by continuous decrease of salt concentration JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 377 LP - 382 DO - 10.1136/jcp.16.4.377 VL - 16 IS - 4 AU - D. Danon Y1 - 1963/07/01 UR - http://jcp.bmj.com/content/16/4/377.abstract N2 - A single volume of 0ยท075 ml. of a 1 in 10 dilution of whole blood in isotonic NaCl solution is introduced into a container cell, two walls of which are made of a dialysing membrane. The container cell is introduced into a test tube of distilled water, placed in an instrument which is essentially a colorimeter with a recorder, between the source of light and the photoelectric cell. Dialysis through the membrane results in a continuous decrease in the salt concentration of the medium surrounding the erythrocytes. The measurement of the degree of haemolysis is based on the increasing transparency of the erythrocyte suspension while haemolysis takes place. Recording this increasing light transmission as a function of time, i.e., as a function of decreasing salt concentration, yields the osmotic fragility curve. The automatically recorded curve is obtained in less than 10 minutes. ER -