RT Journal Article
SR Electronic
T1 Effects of Triton-X-100 upon the mobility of esterases and alkaline phosphatases in disc electrophoresis
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 617
OP 618
DO 10.1136/jcp.19.6.617
VO 19
IS 6
A1 Coutinho, H. B.
A1 Katchburian, E.
A1 Pearse, A. G. E.
YR 1966
UL http://jcp.bmj.com/content/19/6/617.abstract
AB Homogenates of the mid gut and digestive glands of Rhynchosciara angelae were prepared in 40% sucrose, with and without 0·5% Triton-X-100. These were subjected to disc electrophoresis and zymograms for esterases and alkaline phosphatase were prepared. In both groups the effect of Triton-X-100 was to increase the electrophoretic mobility of the enzymes concerned.