TY - JOUR T1 - Modifications in the fluorescence microscopy technique as applied to identification of acid-fast bacilli in tissue and bacteriological material JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 583 LP - 588 DO - 10.1136/jcp.19.6.583 VL - 19 IS - 6 AU - H. Silver AU - A. C. Sonnenwirth AU - N. Alex Y1 - 1966/11/01 UR - http://jcp.bmj.com/content/19/6/583.abstract N2 - The principles and techniques involved in fluorescence microscopy as related to the demonstration of acid-fast bacilli are discussed. The auramine-rhodamine staining method, for both tissue and bacteriological preparations, is described as well as the necessary equipment for both microscopy and photomicroscopy. Fluorescence staining is no more difficult nor time consuming than a Ziehl-Neelsen stain yet it is more effective in demonstrating acid-fast organisms. ER -