PT  - JOURNAL ARTICLE
AU  - Joyce L. Bell
AU  - Susan M. Atkinson
AU  - D. N. Baron
TI  - An AutoAnalyzer method for estimating serum glyceride glycerol using a glycerokinase procedure
AID  - 10.1136/jcp.23.6.509
DP  - 1970 Sep 01
TA  - Journal of Clinical Pathology
PG  - 509--513
VI  - 23
IP  - 6
4099  - http://jcp.bmj.com/content/23/6/509.short
4100  - http://jcp.bmj.com/content/23/6/509.full
SO  - J Clin Pathol1970 Sep 01; 23
AB  - The glyceride glycerol analysis depends, after saponification of triglycerides, on a linked enzymatic procedure using glycerokinase, pyruvate kinase, and lactate dehydrogenase: the final conversion of NADH to NAD+ is followed fluorimetrically. Twenty analyses can be performed per hour on the AutoAnalyzer; recoveries of added triglycerides ranged between 90 and 104%. In a mixed male and female group the normal range for glyceride glycerol was 2·5 to 15·5 mg/100 ml (0·2-1·4 mmol/l) fasting, and 2·5 to 18·0 mg/100 ml (0·2-1·6 mmol/l) postprandially using fresh serum. There was a significant rise postprandially in older men.