RT Journal Article
SR Electronic
T1 An AutoAnalyzer method for estimating serum glyceride glycerol using a glycerokinase procedure
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 509
OP 513
DO 10.1136/jcp.23.6.509
VO 23
IS 6
A1 Bell, Joyce L.
A1 Atkinson, Susan M.
A1 Baron, D. N.
YR 1970
UL http://jcp.bmj.com/content/23/6/509.abstract
AB The glyceride glycerol analysis depends, after saponification of triglycerides, on a linked enzymatic procedure using glycerokinase, pyruvate kinase, and lactate dehydrogenase: the final conversion of NADH to NAD+ is followed fluorimetrically. Twenty analyses can be performed per hour on the AutoAnalyzer; recoveries of added triglycerides ranged between 90 and 104%. In a mixed male and female group the normal range for glyceride glycerol was 2·5 to 15·5 mg/100 ml (0·2-1·4 mmol/l) fasting, and 2·5 to 18·0 mg/100 ml (0·2-1·6 mmol/l) postprandially using fresh serum. There was a significant rise postprandially in older men.