RT Journal Article
SR Electronic
T1 The preservation of gonococci in liquid nitrogen
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 122
OP 123
DO 10.1136/jcp.24.2.122
VO 24
IS 2
A1 Ward, M. E.
A1 Watt, P. J.
YR 1971
UL http://jcp.bmj.com/content/24/2/122.abstract
AB Gonococci suspended in 1% proteose peptone containing 8% glycerol can be snap frozen in liquid nitrogen without detectable loss on thawing. This recovery rate has allowed the use of frozen organisms as the starting inoculum for the bulk growth of gonococci and to preserve gonococci both in urethral pus and after primary subculture for use in studies on gonococcal virulence. The method described for freezing urethral pus on charcoal swabs should make it possible to transport infected specimens from areas lacking adequate laboratory facilities.