RT Journal Article
SR Electronic
T1 An automated microbiological method for the measurement of vitamin B<sub>12</sub>
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 494
OP 498
DO 10.1136/jcp.26.7.494
VO 26
IS 7
A1 R. E. Davis
A1 J. Moulton
A1 A. Kelly
YR 1973
UL http://jcp.bmj.com/content/26/7/494.abstract
AB An automated method for the microbiological assay of vitamin B12 is described. A chloramphenicol-resistant strain of Lactobacillus leichmannii is used as the test organism and this eliminates the need for sterilization or aseptic addition. Precipitation of the serum protein is avoided by dilution of the serum with a glutamic/malic acid solution before heating the preparation to free the vitamin B12. Tests can be set up at a rate of 80 an hour and after incubation they can be read at 160 an hour. By interfacing a programmed electronic calculator directly with the Mecolab M which is used for the assays, results are printed directly in ng/l.