RT Journal Article
SR Electronic
T1 Extraction of serum vitamin B12 for radio-isotopic and Lactobacillus leichmannii assay.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 531
OP 539
DO 10.1136/jcp.28.7.531
VO 28
IS 7
A1 Raven, J L
A1 Robson, M B
YR 1975
UL http://jcp.bmj.com/content/28/7/531.abstract
AB The protein precipitates discarded during the extraction process of the Lactobacillus leichmannii vitamin B12 assay have been shown to contain significant amounts of vitamin B12. This loss of vitamin B12 provide a satisfactory explanation for many of the descrepancies between the serum vitamin B12 values obtained by the L. leichmannii method and the radio-isotopic method of Raven et al (1969). It is possible to produce lower results by the method of Raven et al (1969)by incorporating into that method the L. leichmannii extraction process; it is also possible to produce higher results by the L. leichmannii method using a papain extraction process.