RT Journal Article
SR Electronic
T1 Lability of human creatine kinase isoenzymes at 37 degrees C: a complication of electrophoretic separation.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 834
OP 836
DO 10.1136/jcp.28.10.834
VO 28
IS 10
A1 D A Nealon
A1 A R Henderson
YR 1975
UL http://jcp.bmj.com/content/28/10/834.abstract
AB The activity of the brain specific isoenzyme of creatine kinase is shown to fall off rapidly at 37 degrees C, particularly in the presence of albumin. Dithiothreitol cannot reverse this lability. The implications of this finding suggest that electrophoretic techniques which use incubation methods to detect the brain specific isoenzyme of creatine kinase may underestimate the true activity.