TY - JOUR T1 - Continuous-flow analysis of ammonia in perchloric acid supernate of blood or plasma using an ammonia-selective electrode. JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 1207 LP - 1211 DO - 10.1136/jcp.31.12.1207 VL - 31 IS - 12 AU - G C Moses AU - R J Thibert AU - T F Draisey Y1 - 1978/12/01 UR - http://jcp.bmj.com/content/31/12/1207.abstract N2 - A simple automated method for the estimation of ammonia in perchloric acid supernate of blood or plasma using an ion-selective electrode (Orion Ammonia-selective electrode, Model 95-10) is described. The reliability of the proposed method has been checked against an ion-exchange resin procedure, which has been chosen as a standard procedure. Regression equation and correlation coefficient for the proposed method are y = 0.7 X + 10 and 0.945, respectively, as compared with the chosen standard method. Within-run and between-run precision are 2.1% and 3.5% respectively. The average percent recovery is 97.5% and a tentative range is 13-73 microgram/dl (9-52 micronmol/l) ammonia nitrogen. ER -