RT Journal Article
SR Electronic
T1 Complex nature of serum lysozyme activity: evidence of thermolability in inflammatory bowel disease.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 39
OP 43
DO 10.1136/jcp.31.1.39
VO 31
IS 1
A1 M Ward
A1 W D Mitchell
A1 M Eastwood
YR 1978
UL http://jcp.bmj.com/content/31/1/39.abstract
AB In patients with Crohn's disease and ulcerative colitis, alterations in serum storage temperature produced significant changes in serum lysozyme activity (SLA) as measured by the lysoplate method. This was not the case in healthy controls or in a group with other gastrointestinal disorders. Electrophoretic separation of serum revealed two components of lysozyme-type lytic activity but only one in extracts of gut mucosa, leucocytes, and egg white. The major lytic component of serum migrated towards the cathode and reacted with specific antilysozyme serum, but the minor component which migrated towards the anode did not. Although the cause of this anionic lytic activity is uncertain, it contributes to total serum activity as estimated by any method utilising the lysis of Micrococcus lysodeikticus, and may possibly be related to the observed thermolability.