RT Journal Article
SR Electronic
T1 Use of low ionic strength saline for crossmatching and antibody screening.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 1019
OP 1024
DO 10.1136/jcp.32.10.1019
VO 32
IS 10
A1 Lown, J A
A1 Barr, A L
A1 Davis, R E
YR 1979
UL http://jcp.bmj.com/content/32/10/1019.abstract
AB A totally revised crossmatching and antibody screening procedure has been designed using low ionic strength saline as the suspending medium for red cells in the saline and enzyme phases as well as the antiglobulin test. The replacement of Pasteur pipettes with precision samplers for all serum and red cell dispensing has resulted in improvement in the reproducibility and standardisation of technique. The major advantages are improved sensitivity, shortened incubation time, and simplicity of technical performance.