PT - JOURNAL ARTICLE AU - Dimitracopoulos, G AU - Intzes, C AU - Papavassiliou, J TI - Characteristics of Pseudomonas aeruginosa in relation to laboratory-induced resistance to gentamicin. AID - 10.1136/jcp.32.7.723 DP - 1979 Jul 01 TA - Journal of Clinical Pathology PG - 723--727 VI - 32 IP - 7 4099 - http://jcp.bmj.com/content/32/7/723.short 4100 - http://jcp.bmj.com/content/32/7/723.full SO - J Clin Pathol1979 Jul 01; 32 AB - Pseudomonas aeruginosa strain C2 was habituated to gentamicin by serial passage in broth containing increasing concentrations of the antibiotic and up to 250 microgram/ml. The resistant progenies differed from the parent strain in antibiotic susceptibility to two other aminoglycosides, colonial morphology, lytic phage patterns, phage adsorption, and agglutination with the seven Fisher's antisera. All the progenies failed to grow at 42 degrees C and oxidised glucose in O/F tubes after incubation at 37 degrees C for three days but were catalase- and oxidase-positive. Reversion to the original properties of the parent strain was demonstrated in all cases after 10 serial subcultures in antibiotic-free broth.