TY - JOUR T1 - ELISA for toxoplasma antibody detection: a comparison with other serodiagnostic tests. JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 640 LP - 643 DO - 10.1136/jcp.33.7.640 VL - 33 IS - 7 AU - A Balsari AU - G Poli AU - V Molina AU - M Dovis AU - E Petruzzelli AU - A Boniolo AU - E Rolleri Y1 - 1980/07/01 UR - http://jcp.bmj.com/content/33/7/640.abstract N2 - An ELISA method was developed for the measurement of toxoplasma IgG antibodies in human serum using antigen-coated polystyrene beads as a solid phase and anti human IgG-horse radish peroxidase conjugate as an enzymatic tracer. In order to assess ELISA sensitivity and specificity, a between methods comparison was made using 'conventional' serological tests as reference (dye-test, crossover-linked immunoassay, passive haemagglutination, indirect immunofluorescence). From an analysis of the group classifications obtained some considerations emerged: the ELISA specificity looks comparable with that of the 'reference' tests, as no sample classified as negative by all these tests was ELISA-positive, and vice versa; ELISA appears to correlate better with haemagglutination and immunofluorescence, on the basis of the respective class frequencies; in particular, the number of positives, which is much lower for the dye-test and crossover-linked immunoassay, suggests that a higher sensitivity is reached in the former cases. ER -