RT Journal Article
SR Electronic
T1 False negative hepatitis B surface antigen detection in dialysis patients due to excess surface antigen: postzone phenomenon.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 993
OP 994
DO 10.1136/jcp.33.10.993
VO 33
IS 10
A1 L G Hefter
A1 M A Hix
A1 M Stoner
A1 C B Cook
YR 1980
UL http://jcp.bmj.com/content/33/10/993.abstract
AB Renal dialysis patients are well known to have a high incidence of hepatitis B carrier state. In studying a group of 63 long-term dialysis patients, 10 were found to be positive for hepatitis B surface antigen by radioimmunoassay (RIA). Surprisingly, however, only three of these RIA positive patients were positive by counter immunoelectrophoresis (CIEP). The discrepancy could not be accounted for by the difference in sensitivity of the two methods. The cause for the negative reactions by CIEP in seven patients was found to be the marked excess surface antigen in these sera which produced false negative results by the postzone phenomenon. After dilution all seven sera were positive by CIEP, requiring a dilution up to 1/20 to produce a positive result. Also, all seven sera were positive by the less sensitive Ouchterlony double diffusion.