RT Journal Article SR Electronic T1 Human pulmonary macrophages: the relationship of smoking to the presence of sea blue granules and surfactant turnover. JF Journal of Clinical Pathology JO J Clin Pathol FD BMJ Publishing Group Ltd and Association of Clinical Pathologists SP 738 OP 743 DO 10.1136/jcp.33.8.738 VO 33 IS 8 A1 P N Plowman A1 R J Flemans YR 1980 UL http://jcp.bmj.com/content/33/8/738.abstract AB Human pulmonary macrophages from heavy smokers usually (17/21 cases) contain sea blue inclusion material in the cytoplasm. In non-smokers, sea blue material is rarely evident (1/21 cases). The sea blue material observed has the same properties as sea blue material identified in other conditions (viz, Sudan black and PAS positivity and autofluorescence). Evidence is presented which suggests that pulmonary macrophages from smokers contain extra surfactant and that it is this phospholipid complex that accounts for the sea blue reaction observed.