RT Journal Article
SR Electronic
T1 Detection of Bacteroides fragilis and Bacteroides melaninogenicus by direct immunofluorescence.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 1189
OP 1192
DO 10.1136/jcp.33.12.1189
VO 33
IS 12
A1 M Labbé
A1 N Delamare
A1 F Pepersack
A1 F Crokaert
A1 E Yourassowsky
YR 1980
UL http://jcp.bmj.com/content/33/12/1189.abstract
AB A new diagnostic kit, which contains a polyvalent antiserum for either Bacteroides fragilis or Bacteroides melaninogenicus, was tested for reliability and specificity on 146 clinical samples of different origin. A correlation between the culture and immunofluorescence was observed for B. fragilis in 87.39% of cases and for B. melaninogenicus in 81.48% of cases. When pure cultures were tested, aerobically as well as anaerobically, false-positive reactions were observed with staphylococci and Clostridium ramosum spores. The well-defined morphology of these bacteria and spores allows for the elimination of any diagnostic error. The method is rapid, and the margin for error is limited. The test gives a semiquantitative idea of the number of bacteroides organisms present in the clinical specimens even in the presence of a mixed flora.