RT Journal Article
SR Electronic
T1 Cell structure and percent viability by a slide centrifuge technique.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 191
OP 194
DO 10.1136/jcp.35.2.191
VO 35
IS 2
A1 M G Fitzgerald
A1 C S Hosking
YR 1982
UL http://jcp.bmj.com/content/35/2/191.abstract
AB It was found that a slide centrifuge (Cytospin) preparation of a cell suspension allowed a reliable assessment of not only cell structure but also the percentage of non-viable cells. The non-viable cells appeared as "smear" cells and paralleled in number the cells taking up trypan blue. Direct experiment showed the unstained viable cells in a trypan blue cell suspension remained intact in a Cytospin preparation while the cells taking up trypan blue were the "smear" cells. The non-viability of the "smear" cells was confirmed by their inability to survive in culture.