RT Journal Article
SR Electronic
T1 Cultural method for large-scale screening for Chlamydia trachomatis genital infection.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 712
OP 718
DO 10.1136/jcp.34.7.712
VO 34
IS 7
A1 Mallinson, H
A1 Sikotra, S
A1 Arya, O P
YR 1981
UL http://jcp.bmj.com/content/34/7/712.abstract
AB Idoxuridine-treated McCoy cells grown as monolayers in 96 well microplates provide a convenient method for the isolation of Chlamydia trachomatis. Staining of infected monolayers with periodic acid-Schiff reagent (PAS) allows easy recognition of C trachomatis inclusions without the need for dark-ground microscopy. By this method 384 clinical specimens can be examined concurrently. It is sufficiently sensitive to form the basis of a chlamydial culture service for patients attending Sexually Transmitted Diseases (STD) Clinics.