RT Journal Article
SR Electronic
T1 Use of whole blood in the measurement of neutrophil migration.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 158
OP 162
DO 10.1136/jcp.34.2.158
VO 34
IS 2
A1 Al-Hadithy, H
A1 Addison, I E
A1 Goldstone, A H
YR 1981
UL http://jcp.bmj.com/content/34/2/158.abstract
AB A whole-blood method was used for measuring neutrophil migration in micropore membrane. The method is reproducible (coefficient of variation (CV) 10.3% and 9.8% in two normal individuals tested repeatedly) and can be performed on an 0.25 ml lithium heparin blood sample. Migration is independent of leucocyte and erythrocyte counts and is comparable with that obtained for separated cells. As in separated cell techniques, cytochalasin and casein respectively inhibit and stimulate neutrophil migration. The technique is of value when testing large numbers of samples and when only small volumes of blood can be obtained, for example, in neonates.