PT  - JOURNAL ARTICLE
AU  - Räisänen, S
AU  - Suni, J I
AU  - Vaheri, A
TI  - Mycoplasma pneumoniae protein involved in the antibody response in human infection.
AID  - 10.1136/jcp.37.10.1129
DP  - 1984 Oct 01
TA  - Journal of Clinical Pathology
PG  - 1129--1133
VI  - 37
IP  - 10
4099  - http://jcp.bmj.com/content/37/10/1129.short
4100  - http://jcp.bmj.com/content/37/10/1129.full
SO  - J Clin Pathol1984 Oct 01; 37
AB  - Antigen from purified Mycoplasma pneumoniae organisms treated with Tween-80-ether was used in a solid phase enzyme immunoassay and compared with the conventional lipid containing complement fixation antigen for measuring antibodies in sera from patients with aseptic or bacterial meningitis or with apparent M pneumoniae infection. In immunoblotting of the enzyme immunoassay antigen, enzyme immunoassay positive sera detected a polypeptide at Mr = 180.000-200.000, while enzyme immunoassay negative sera whether positive or negative in the complement fixation test did not. These results indicate that the enzyme immunoassay antigen containing the high molecular weight polypeptide can be used to measure M pneumoniae antibodies more specifically than the conventional lipid containing complement fixation antigen.