RT Journal Article
SR Electronic
T1 Mycoplasma pneumoniae protein involved in the antibody response in human infection.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 1129
OP 1133
DO 10.1136/jcp.37.10.1129
VO 37
IS 10
A1 Räisänen, S
A1 Suni, J I
A1 Vaheri, A
YR 1984
UL http://jcp.bmj.com/content/37/10/1129.abstract
AB Antigen from purified Mycoplasma pneumoniae organisms treated with Tween-80-ether was used in a solid phase enzyme immunoassay and compared with the conventional lipid containing complement fixation antigen for measuring antibodies in sera from patients with aseptic or bacterial meningitis or with apparent M pneumoniae infection. In immunoblotting of the enzyme immunoassay antigen, enzyme immunoassay positive sera detected a polypeptide at Mr = 180.000-200.000, while enzyme immunoassay negative sera whether positive or negative in the complement fixation test did not. These results indicate that the enzyme immunoassay antigen containing the high molecular weight polypeptide can be used to measure M pneumoniae antibodies more specifically than the conventional lipid containing complement fixation antigen.