PT  - JOURNAL ARTICLE
AU  - Qureshi, M Y
AU  - Smith, S M
AU  - Murphy, G M
TI  - Colorimetric enzymatic measurement of serum total 3 alpha-hydroxy bile acid concentrations without extraction.
AID  - 10.1136/jcp.37.3.317
DP  - 1984 Mar 01
TA  - Journal of Clinical Pathology
PG  - 317--320
VI  - 37
IP  - 3
4099  - http://jcp.bmj.com/content/37/3/317.short
4100  - http://jcp.bmj.com/content/37/3/317.full
SO  - J Clin Pathol1984 Mar 01; 37
AB  - A new method for the simpler colorimetric assay of serum total 3 alpha-hydroxy bile acid concentrations without prior extraction has been investigated. The lowest concentration that could be reliably measured was 3 mumol/l and the results obtained were in agreement with those obtained using extraction and the spectrofluorimetric enzymatic assay (y = 1.007x - 1.86, r = 0.99, n = 36). Results for 20 normal sera collected after an overnight fast were 6.2 +/- 5.1 mumol/l (mean +/- SD) and in 20 normal plasma randomly collected were 4.9 +/- 4.2 mumol/l. This new assay is comparable in specificity and sensitivity to the older spectrofluorimetric enzymatic assays and convenient for routine use.