TY - JOUR T1 - Colorimetric enzymatic measurement of serum total 3 alpha-hydroxy bile acid concentrations without extraction. JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 317 LP - 320 DO - 10.1136/jcp.37.3.317 VL - 37 IS - 3 AU - M Y Qureshi AU - S M Smith AU - G M Murphy Y1 - 1984/03/01 UR - http://jcp.bmj.com/content/37/3/317.abstract N2 - A new method for the simpler colorimetric assay of serum total 3 alpha-hydroxy bile acid concentrations without prior extraction has been investigated. The lowest concentration that could be reliably measured was 3 mumol/l and the results obtained were in agreement with those obtained using extraction and the spectrofluorimetric enzymatic assay (y = 1.007x - 1.86, r = 0.99, n = 36). Results for 20 normal sera collected after an overnight fast were 6.2 +/- 5.1 mumol/l (mean +/- SD) and in 20 normal plasma randomly collected were 4.9 +/- 4.2 mumol/l. This new assay is comparable in specificity and sensitivity to the older spectrofluorimetric enzymatic assays and convenient for routine use. ER -