PT  - JOURNAL ARTICLE
AU  - Supran, E M
AU  - Boxall, E H
AU  - Craske, J
AU  - Hart, R J
AU  - Vandervelde, E M
AU  - Gardner, P S
TI  - Enzyme-linked immunosorbent assay (ELISA) for the detection of hepatitis Be antigen and antibody: report of a field trial.
AID  - 10.1136/jcp.36.5.581
DP  - 1983 May 01
TA  - Journal of Clinical Pathology
PG  - 581--585
VI  - 36
IP  - 5
4099  - http://jcp.bmj.com/content/36/5/581.short
4100  - http://jcp.bmj.com/content/36/5/581.full
SO  - J Clin Pathol1983 May 01; 36
AB  - A field trial of an enzyme-linked immunosorbent assay (ELISA) for the detection of the hepatitis Be markers is reported. It is simple to perform, is designed to be read by eye and does not require any expensive apparatus. When compared with a commercially available RIA kit for the detection of the same markers, ELISA was shown to be as sensitive as RIA for the detection of anti-HBe but slightly less sensitive for the detection of HBeAg. However if all specimens negative for both HBeAg and anti-HBe by ELISA are considered to be potentially infectious, the ELISA should prove to be as useful as RIA for determining the "e" status of HBsAg-positive patients and, therefore, provide a reliable indication of the risk of secondary spread of hepatitis B infection to contacts by needle stick accident, close personal contact or perinatal transmission.