PT  - JOURNAL ARTICLE
AU  - G Mascart
AU  - F Bertrand
AU  - P Mascart
TI  - Comparative study of subculture, Gram staining and acridine orange staining for early detection of positive blood cultures.
AID  - 10.1136/jcp.36.5.595
DP  - 1983 May 01
TA  - Journal of Clinical Pathology
PG  - 595--597
VI  - 36
IP  - 5
4099  - http://jcp.bmj.com/content/36/5/595.short
4100  - http://jcp.bmj.com/content/36/5/595.full
SO  - J Clin Pathol1983 May 01; 36
AB  - In view of the importance of a rapid aetiological diagnosis in septicaemia, we compared the results of subculture, Gram staining and acridine orange staining in the detection of positive blood cultures. The study was based on 1013 blood cultures of which 138 were positive by culture. The three techniques were applied 12 h after the specimen was taken in 210 instances, at 24 h in 540 instances and after 48 h in 525. We were able to demonstrate the value of direct examination. Staining with acridine orange yields more positive results than Gram staining and is also simpler.