PT  - JOURNAL ARTICLE
AU  - E F Rimmer
AU  - C Turberville
AU  - M A Horton
TI  - Human mast cells detected by monoclonal antibodies.
AID  - 10.1136/jcp.37.11.1249
DP  - 1984 Nov 01
TA  - Journal of Clinical Pathology
PG  - 1249--1255
VI  - 37
IP  - 11
4099  - http://jcp.bmj.com/content/37/11/1249.short
4100  - http://jcp.bmj.com/content/37/11/1249.full
SO  - J Clin Pathol1984 Nov 01; 37
AB  - We report the establishment of seven mouse-mouse hybridoma cell lines secreting monoclonal antibodies with specificity for granule components of all human mast cells. Reactivity is directed against a molecule which is also found intracytoplasmically in human mature small intestinal enterocytes, liver parenchymal cells, and kidney proximal tubule epithelial cells. No reactivity of these antibodies was found with any other human or animal cell type examined. In particular, the antibodies did not react with basophils or other haemopoietic cell types. This study shows the potential of specific monoclonal antibodies as a tool for identifying and enumerating infiltrating mast cells in tissues. Such antibodies should be of value in investigations into the role of the mast cell in immunological reactions and hypersensitivity diseases.