PT - JOURNAL ARTICLE AU - J P Burnie AU - R C Matthews AU - A Fox AU - S Tabaqchali TI - Use of immunoblotting to identify antigenic differences between the yeast and mycelial phases of Candida albicans. AID - 10.1136/jcp.38.6.701 DP - 1985 Jun 01 TA - Journal of Clinical Pathology PG - 701--706 VI - 38 IP - 6 4099 - http://jcp.bmj.com/content/38/6/701.short 4100 - http://jcp.bmj.com/content/38/6/701.full SO - J Clin Pathol1985 Jun 01; 38 AB - Western blotting was applied to the analysis of Candida albicans in the yeast and mycelial phases in an attempt to recognise mycelial specific antigens which might be of serodiagnostic value. The antisera were prepared in rabbits by immunising them with pressates of C albicans type A NCTC 3153 in the yeast phase or the mycelial phase. These were blotted against C albicans in the yeast and mycelial phases and the yeast phase of C parapsilosis, C krusei, C tropicalis, and Torulopsis glabrata. Cross reactivity was greatest against C parapsilosis. One yeast specific mannoprotein was identified with a molecular weight of 49 000. No mycelial specific antigens could be identified.