RT Journal Article SR Electronic T1 Assessment of DNA content and cell cycle distribution of erythroid and myeloid cells from bone marrow. JF Journal of Clinical Pathology JO J Clin Pathol FD BMJ Publishing Group Ltd and Association of Clinical Pathologists SP 1120 OP 1124 DO 10.1136/jcp.41.10.1120 VO 41 IS 10 A1 Hodgetts, J A1 Hoy, T G A1 Jacobs, A YR 1988 UL http://jcp.bmj.com/content/41/10/1120.abstract AB A method is described for the measurement of DNA index and cell cycle distribution in purified erythroid and myeloid populations from human bone marrow. Erythroid cells were prepared after complement mediated lysis of non-erythroid marrow cells. Myeloid cells were obtained by fluorescence activated cell sorting by forward and wide angle light scatter. Mononuclear marrow cells were prepared with a density gradient. Nuclei prepared from the separated populations were stained with propidium iodide. Myeloid cells had a higher DNA index than erythroid cells, and the mononuclear preparation had an intermediate value. There were more erythroid than myeloid cells in the S and G2M phases of the cell cycle. These lineage differences are particularly relevant when considering data derived from unseparated bone marrow cells, and further experiments are needed to determine the origin of these anomalies.