%0 Journal Article %A M L Friedlander %A D R Bell %A J Leary %A R A Davey %T Comparison of western blot analysis and immunocytochemical detection of P-glycoprotein in multidrug resistant cells. %D 1989 %R 10.1136/jcp.42.7.719 %J Journal of Clinical Pathology %P 719-722 %V 42 %N 7 %X A sensitive immunocytochemical technique was developed to detect a 170,000 dalton cell membrane glycoprotein (P-gp) in cell lines resistant to vincristine and vinblastine with varying degrees of resistance. P-gp was shown very clearly using the C219 monoclonal antibody and immunocytochemical detection with either antialkaline phosphate or peroxidase-antiperoxidase with silver gold intensification. There was good correlation between the results obtained with immunocytochemical detection of P-gp in single cells and Western blot analysis. The technique is easily performed and can detect P-gp in relatively small numbers of cells that Western blot analysis could miss and is suitable for clinical application. %U https://jcp.bmj.com/content/jclinpath/42/7/719.full.pdf