PT  - JOURNAL ARTICLE
AU  - M L Friedlander
AU  - D R Bell
AU  - J Leary
AU  - R A Davey
TI  - Comparison of western blot analysis and immunocytochemical detection of P-glycoprotein in multidrug resistant cells.
AID  - 10.1136/jcp.42.7.719
DP  - 1989 Jul 01
TA  - Journal of Clinical Pathology
PG  - 719--722
VI  - 42
IP  - 7
4099  - http://jcp.bmj.com/content/42/7/719.short
4100  - http://jcp.bmj.com/content/42/7/719.full
SO  - J Clin Pathol1989 Jul 01; 42
AB  - A sensitive immunocytochemical technique was developed to detect a 170,000 dalton cell membrane glycoprotein (P-gp) in cell lines resistant to vincristine and vinblastine with varying degrees of resistance. P-gp was shown very clearly using the C219 monoclonal antibody and immunocytochemical detection with either antialkaline phosphate or peroxidase-antiperoxidase with silver gold intensification. There was good correlation between the results obtained with immunocytochemical detection of P-gp in single cells and Western blot analysis. The technique is easily performed and can detect P-gp in relatively small numbers of cells that Western blot analysis could miss and is suitable for clinical application.