RT Journal Article
SR Electronic
T1 Comparison of western blot analysis and immunocytochemical detection of P-glycoprotein in multidrug resistant cells.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 719
OP 722
DO 10.1136/jcp.42.7.719
VO 42
IS 7
A1 M L Friedlander
A1 D R Bell
A1 J Leary
A1 R A Davey
YR 1989
UL http://jcp.bmj.com/content/42/7/719.abstract
AB A sensitive immunocytochemical technique was developed to detect a 170,000 dalton cell membrane glycoprotein (P-gp) in cell lines resistant to vincristine and vinblastine with varying degrees of resistance. P-gp was shown very clearly using the C219 monoclonal antibody and immunocytochemical detection with either antialkaline phosphate or peroxidase-antiperoxidase with silver gold intensification. There was good correlation between the results obtained with immunocytochemical detection of P-gp in single cells and Western blot analysis. The technique is easily performed and can detect P-gp in relatively small numbers of cells that Western blot analysis could miss and is suitable for clinical application.