PT  - JOURNAL ARTICLE
AU  - J McCorriston
TI  - New method for preserving cytology specimens.
AID  - 10.1136/jcp.42.10.1101
DP  - 1989 Oct 01
TA  - Journal of Clinical Pathology
PG  - 1101--1103
VI  - 42
IP  - 10
4099  - http://jcp.bmj.com/content/42/10/1101.short
4100  - http://jcp.bmj.com/content/42/10/1101.full
SO  - J Clin Pathol1989 Oct 01; 42
AB  - A method of freezing cytological material for long term storage, based on a modification of that used in tissue typing for the storage of lymphocytes, was developed. The method entails the centrifugation of the specimen, adding 1 ml AB or fetal calf serum and 1 ml 20% dimethyl sulphoxide aliquoted into 2 ml tubes, and storage at -70 degrees C. Cytological detail was well preserved in a variety of samples including fine needle aspirates, urine, and bronchial brushings. Three dimensional architecture was also preserved. The method is practical, easy to perform, and allows retrospective studies to be undertaken with the subsequent use of a variety of special staining techniques, including immunocytochemistry.