TY - JOUR T1 - New method for preserving cytology specimens. JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 1101 LP - 1103 DO - 10.1136/jcp.42.10.1101 VL - 42 IS - 10 AU - J McCorriston Y1 - 1989/10/01 UR - http://jcp.bmj.com/content/42/10/1101.abstract N2 - A method of freezing cytological material for long term storage, based on a modification of that used in tissue typing for the storage of lymphocytes, was developed. The method entails the centrifugation of the specimen, adding 1 ml AB or fetal calf serum and 1 ml 20% dimethyl sulphoxide aliquoted into 2 ml tubes, and storage at -70 degrees C. Cytological detail was well preserved in a variety of samples including fine needle aspirates, urine, and bronchial brushings. Three dimensional architecture was also preserved. The method is practical, easy to perform, and allows retrospective studies to be undertaken with the subsequent use of a variety of special staining techniques, including immunocytochemistry. ER -