RT Journal Article
SR Electronic
T1 Rapid diagnosis of whooping cough using monoclonal antibody.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 573
OP 575
DO 10.1136/jcp.41.5.573
VO 41
IS 5
A1 P C Boreland
A1 S H Gillespie
A1 L A Ashworth
YR 1988
UL http://jcp.bmj.com/content/41/5/573.abstract
AB A counterimmunoelectrophoresis (CIE) method for antigen detection using monoclonal antibody was assessed for its ability to aid in the rapid diagnosis of Bordetella pertussis in 59 patients. A positive diagnosis from a combination of results from tests of serum and urine was obtained in 51 (87%) of cases. For sera, CIE had a sensitivity of 85% and a specificity of 94%; for urine samples the sensitivity was 81% and a specificity of 100%. Antigen detection by CIE is simple to perform and yields results on the same day, thus allowing treatment to begin at an early stage.