PT  - JOURNAL ARTICLE
AU  - K A Pulford
AU  - W N Erber
AU  - J A Crick
AU  - I Olsson
AU  - K J Micklem
AU  - K C Gatter
AU  - D Y Mason
TI  - Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells.
AID  - 10.1136/jcp.41.8.853
DP  - 1988 Aug 01
TA  - Journal of Clinical Pathology
PG  - 853--860
VI  - 41
IP  - 8
4099  - http://jcp.bmj.com/content/41/8/853.short
4100  - http://jcp.bmj.com/content/41/8/853.full
SO  - J Clin Pathol1988 Aug 01; 41
AB  - A monoclonal antibody, NP57, was produced and used against the neutrophil granule protein elastase, which selectively stain neutrophils in cryostat and paraffin wax sections. The antibody stains neutrophils and a subpopulation of monocytes in blood smears and neutrophil precursors in bone marrow smears, and gives positive reactions with the cell lines HL60 and U-937. It labelled the blast cells in 68% of cases of acute myeloid leukaemia (M1-M5) but was unreactive with all cases of lymphoid leukaemias. Most of the elastase negative myeloid leukaemias were labelled by monoclonal anti-myeloperoxidase (antibody MPO-7) as were cells from the promyelocytic line HL60. No cases of myeloid leukaemia showed the opposite pattern--that is elastase positive, myeloperoxidase negative, suggesting that the production of myeloperoxidase precedes the onset of elastase synthesis during myeloid maturation. The anti-elastase antibody NP57 is a useful addition to the range of monoclonal antibodies available for the differential diagnosis of acute leukaemia by alkaline phosphatase-antialkaline phosphatase (APAAP) labelling of cell smears; it may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections.