TY - JOUR T1 - Rapid assessment of haemophilia A carrier state by non-invasive techniques using the polymerase chain reaction. JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 505 LP - 507 DO - 10.1136/jcp.43.6.505 VL - 43 IS - 6 AU - J Ball AU - L J Warnock AU - F E Preston Y1 - 1990/06/01 UR - http://jcp.bmj.com/content/43/6/505.abstract N2 - The polymerase chain reaction (PCR) was used to amplify two polymorphic regions in the factor VIII gene. In vitro synthesis of DNA was achieved using samples obtained from buccal cells, urine, and hair follicles in addition to purified genomic and crude DNA samples prepared from whole blood. Female members of two kindreds affected with haemophilia A were assessed for carrier state using direct restriction fragment length polymorphism analysis of amplified gene products in the Bc1I and XbaI regions. It is concluded that this is a non-invasive, rapid, and inexpensive technique for carrier detection. ER -