PT  - JOURNAL ARTICLE
AU  - A M Hirschl
AU  - B J Rathbone
AU  - J I Wyatt
AU  - J Berger
AU  - M L Rotter
TI  - Comparison of ELISA antigen preparations alone or in combination for serodiagnosing Helicobacter pylori infections.
AID  - 10.1136/jcp.43.6.511
DP  - 1990 Jun 01
TA  - Journal of Clinical Pathology
PG  - 511--513
VI  - 43
IP  - 6
4099  - http://jcp.bmj.com/content/43/6/511.short
4100  - http://jcp.bmj.com/content/43/6/511.full
SO  - J Clin Pathol1990 Jun 01; 43
AB  - The immunoglobulin G antibody response to Helicobacter pylori was assessed in 78 patients with non-ulcer dyspepsia using five different antigen preparations. All patients were endoscoped and biopsied. The H pylori state was determined histologically on at least two endoscopic biopsy specimens using a modified Giemsa stain. The ultracentrifuged cell sonicate, acid glycine extract, and 120 kilodalton protein antigens were specific in diagnosing infection (95-98%), but had only moderate sensitivity (70-84%). By mixing either of the two complex antigens with the 120 kilodalton protein, the sensitivity of the test was increased to 97% without affecting the high specificity. The combination of ultracentrifuged sonicate or acid glycine extract with the 120 kilodalton protein therefore seems to be superior to the individual antigen preparations and is particularly suitable for the serodiagnosis of H pylori infection.